Rare Earth Desorption Test with Citrate and Bicarbonate GTO_data_deposit_092017_Desorption.xlsx

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.

Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis. Excel file detailing results of the bicarbonate and citrate desorption experiments. Plots of results included.

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Field Value
Citation Date 2017-06-01T06:00:00+00:00

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Original ID f0000000-58cc-4372-a567-000000000965
Index Date 2018-06-14T16:28:49+00:00
Original Format ISO-USGIN
Original Version 1.2

Author

Name Yongqin Jiao
Position primary contact
Organization Lawrence Livermore National Laboratory
Email jiao1@llnl.gov

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